Transient triple TET (tet1/2/3/) knockouts in zebrafish

In our latest work, Sam Ross provides a detailed protocol for the generation of triple TET zebrafish CRISPants (F0 CRISPR/Cas9 KOs) and their subsequent molecular analyses by reduced representation bisulfite sequencing (RRBS). The full protocol can be accessed here, while the entire book (TET proteins and DNA demethylation, Methods and Protocols) can be obtained through the Springer website.